Environmental DNA

- eDNA: a research area in focus at the Department of Environmental Science (a "Strategic Growth Area", SGA)

Graphical representation of DNA sequences of mobile genetic elements from different environmental phyla

Microorganisms are present in practically all parts of the environment as well as in tissues of living organisms, where they may greatly influence the life of humans and the environment in multiple ways. DNA sequencing analysis of microbial DNA sampled from these environments constitutes a great opportunity to study the role and ecology of these organisms and get insight into their metabolism, function and evolutionary relationship. The Section of Microbiology and Biotechnology houses state-of-the-art sequencing facilities to support participation in all types of projects involving analysis of microbial eDNA. This includes identification of single microorganisms and entire microbial communities in various matrices, their expression of genes relevant for provision of important ecosystem services and health of humans, animals and plants. In addition, the eDNA SGA works with development of techniques for detection and monitoring of DNA from higher organisms.

Illumina MiSeq- and NextSeq-sequencing platforms for analysing eDNA

Main research areas

  • Study microbial eDNA from various environments (soil, water, atmosphere, the arctics, animal gut, food production) in order to understand their ecology and function in these settings.
  • Through DNA sequencing investigate genetic drivers of bacterial evolution by comparing metagenomic contents of between pristine and evolutionary challenged environments.
  • Characterization of bacterial communities by 16S rRNA gene analysis, whole genome sequencing of key microbial organisms (vira, bacteria, fungi and protozoans) and transcriptome analysis related with diseases in humans, animals and plants and degradation of pollutants.
  • Use eDNA analysis to understand the role of microbes in provision of ecosystem services like biogeochemical cycling of nutrients, degradation of organic micro pollutants, suppression of plant pests.
  • Detection and risk assessment of hazardous microorganism in air, soil, water and food in relation to human and environmental health.
  • Mobilomics: Sequence-based studies on mobile genetic elements such as plasmids, bacteriophages and IS elements.

Examples of recent and ongoing projects 

Testing a bacterial strain for resistance to antibiotics

Publications

Environmental DNA - publications within the research area.

Click to expand publications list

Tidsskriftartikel

Poster

Udgivelser på nettet - Net-publikation

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Environmental DNA - persons involved

Toke Bang-Andreasen, Videnskabelig assistent, Institut for Miljøvidenskab - Environmental microbiology & biotechnology
Lea Ellegaard-Jensen, Forsker, Institut for Miljøvidenskab - Environmental microbiology & biotechnology
Ulrich Bay Gosewinkel, Seniorforsker, Institut for Miljøvidenskab - Environmental microbiology & biotechnology
Lars Hestbjerg Hansen, Sektionsleder, Professor, Institut for Miljøvidenskab - Environmental microbiology & biotechnology
Niels Bohse Hendriksen, Seniorforsker, Institut for Miljøvidenskab - Environmental microbiology & biotechnology
Carsten Suhr Jacobsen, Institutleder, Professor, Institut for Miljøvidenskab
Anders Johansen, Seniorforsker, Institut for Miljøvidenskab - Environmental microbiology & biotechnology
Witold Kot, Forsker, Institut for Miljøvidenskab - Environmental microbiology & biotechnology
Tue Kjærgaard Nielsen, Postdoc, Institut for Miljøvidenskab - Environmental microbiology & biotechnology
Anne Winding, Viceinstitutleder, seniorforsker, Institut for Miljøvidenskab